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benzoylecgonine (BZE) and ecgonine methyl ester (EME) in human urine has been established. Here, we investigated the use of on-line sample extraction coupled to rapid chromatography systems for tandem mass analysis of COC, EMF, and BZE in human urine. Using this method, sample preparation consisted of a …

Cocaine, Benzoylecgonine and Ecgonine Methyl Ester Determinations in Post Mortem Human Urine and Blood by Gas Chromatography and Capillary Gas Chromatography after “Extrelut®” Extraction | SpringerLink. Developments in Analytical Methods in Pharmaceutical, Biomedical, and Forensic Sciences. 80144-9 Ecgonine methyl ester [Presence] in Urine by Screen method Active Part Description. LP28530-1 Ecgonine methyl ester Ecgonine methyl ester (EME) is a major metabolite of cocaine, a wide spread illicit drug of abuse. [PMID: 7876811] Source: Regenstrief LOINC, PMID: 7876811.

Ecgonine methyl ester in urine

It has a role as a central nervous system depressant, a peripheral nervous system drug, an analgesic, a metabolite, an opioid analgesic and a mouse metabolite. It is a tropane alkaloid, a methyl ester and a tertiary amino compound. It derives from an ecgonine. Ecgonine methyl ester is a major urinary metabolite of cocaine, a popular illicit drug well known as "crack" or the free base form of cocaine that can be smoked. This stable-labeled internal standard is suitable for use in cocaine testing or isotope dilution methods for GC/MS or LC/MS applications in urine drug testing, clinical toxicology or forensic analysis.

Here, we investigated the use of on-line sample extraction coupled to rapid chromatography systems for tandem mass analysis of COC, EMF, and BZE in human urine.

In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. After an initial quantitation (t = 0), these specimens were

Institute for In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. After an initial quantitation (t = 0), these specimens were The main metabolites are benzoylecgonine (BE) and ecgonine methyl ester (EME). Other metabolites such as norcocaine and cocaethylene have been analyzed in both blood and urine, but most laboratories use methods that detect cocaine, BE and EME. The excretion kinetics of cocaine (C) and its two major metabolites, benzoylecgonine (BZ) and ecgonine methyl ester (EME), were determined by collecting all urine for 30 h from 5 cocaine users (subjects C, D, E, F, and G) given bolus doses followed by exponential cocaine infusions that delivered doses of 253 (subject C), 444 (subjects D, E, and F), and 700 mg (subject G). The two major urinary metabolites of cocaine are benzoylecgonine (BE) and ecgonine methyl ester (EME). The major advantage of BE screening is that many commercial immunoassays are designed to detect BE. On the other hand, EME is more amenable to gas chromatographic screening.

The principal urine metabolites are benzoyl-ecgonine and ecgonine-methyl ester . Only small amounts of cocaine are excreted in the urine. The elimination half-life

These results indicate that ecgonine methyl ester is a prominent urinary metabolite after street use of cocaine, which primarily involves intranasal application and intravenous injection rather than ingestion. In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. This is a comment on "Sensitive, selective, and rapid procedure for the quantitation of ecgonine methyl ester in urine." J Anal Toxicol. 1991 Nov-Dec;15(6):345. This is a comment on "Sensitive, selective, and rapid procedure for the quantitation of ecgonine methyl ester in urine." J Anal Toxicol.

[82] performed a thorough study on subjects with a previous history of cocaine use. The latter route is particularly important since it is globally a route of detoxification that accounts for as much as 90% of the dose in humans, who excrete high urinary concentrations of benzoylecgonine (2), ecgonine methyl ester (3), and ecgonine (4). A rapid, rugged, and highly specific assay for the quantitation of cocaine (COC) and especially its primary metabolites benzoylecgonine (BZE) and ecgonine methyl ester (EME) in human urine … Urine or plasma or serum is spiked with deuterated internal standards cocaine-d3, benzoylecgonine-d3, ecgonine methyl ester-d3, and cocaethylene-d3 and buffered with phosphate buffer. The drugs in the sample are extracted by cation-exchange solid phase extraction. In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. After an initial quantitation (t = 0), these specimens were split and stored at either 4 degrees C or -20 degrees C. Cocaine, Benzoylecgonine and Ecgonine Methyl Ester Determinations in Post Mortem Human Urine and Blood by Gas Chromatography and Capillary Gas Chromatography after “Extrelut®” Extraction | SpringerLink.
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The major advantage of BE screening is that many commercial immunoassays are designed to detect BE. On the other hand, EME is more amenable to gas chromatographic screening. To ascertain the merits … In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. Long-term stability of ecgonine methyl ester in urine. Vasiliades J. Erratum in J Anal Toxicol 1993 Oct;17(6):9.

Cocaine is rapidly cleared from plasma, but variably, at 20 to 30 mL/min/kg. Elimination half-life of cocaine is similarly variable, Froldi R., Gambaro V., Groppi A. (1987) Cocaine, Benzoylecgonine and Ecgonine Methyl Ester Determinations in Post Mortem Human Urine and Blood by Gas Chromatography and Capillary Gas Chromatography after “Extrelut®” Extraction. zoylecgonine (BZE) and ecgonine methyl ester (EME). Both compounds remain detectable 4-6 times longer than COC in urine and plasma.
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ECGONINE METHYL ESTER: First major axis-component or analyte Property: PRTHR: Second major axis-property observed (e.g., mass vs. substance) Time Aspect: PT: Third major axis-timing of the measurement (e.g., point in time vs 24 hours) System: URINE

These are organic compounds containing the nitrogenous bicyclic alkaloid parent N-Methyl-8-azabicyclo[3.2.1]octane. Ecgonine methyl ester is a major metabolite of cocaine. Pseudoecgonine methyl ester and ecgonine methyl ester are formed as artifacts from the effect of sodium bicarbonate and methanol in the injection port of a gas chromatograph. Esters Toxicology screen, general (procedure) Urine ecgonine methyl ester.

Ecgonine ethyl ester solution 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®; CAS Number: 70939-97-8; EC Number: 200-835-2; Linear Formula: C11H19NO3; find Supelco-E-019 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

ZINC34416009. Ecgonine Ethyl Ester 0.1 mg/ml in Acetonitrile. 2,2-DIFLUORO-BENZO[1,3]DIOXOLE-4-BORONICACID. Q27292836. ETHYL (1R,2R,3S,5S)-3-HYDROXY-8-METHYL-8-AZABICYCLO[3.2.1]OCTANE-2-CARBOXYLATE. 8-Azabicyclo(3.2.1)octane-2-carboxylic acid, 3-hydroxy-8-methyl-, ethyl ester, (R-(exo,exo))- 1988-09-01 · A method for the simultaneous analysis of cocaine (COC), derivatized benzoylecgonine (BE), and derivatized ecgonine methyl ester (EME) has been developed.

A sensitive and specific bioanalyt- Ambre JJ, Ruo TI, Smith GL, Backes D, Smith CM 1982 Ecgonine methyl ester, a major metabolite of cocaine. J Anal Toxicol 6 : 26–29. CAS Article Google Scholar Recently, ecgonine methyl ester (methylecgonine) has been shown to be major urinary metabolite of cocaine in man after oral administration. These results indicate that ecgonine methyl ester is a prominent urinary metabolite after street use of cocaine, which primarily involves intranasal application and intravenous injection rather than ingestion. In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. This is a comment on "Sensitive, selective, and rapid procedure for the quantitation of ecgonine methyl ester in urine." J Anal Toxicol.